Promoter studies.:

First I will demonstrate some of the tools available on the web. I will also mention some tools you should install locally.

1- Please try your project gene, first 2000 bp and later with 3000 bp

http://www.cbs.dtu.dk/services/Promoter/

http://fastlink.nih.gov/molbio/proscan/index.html

http://www.fruitfly.org/seq_tools/promoter.html

http://genes.mit.edu/McPromoter.html

2- Comparative Genomics approach:

Align 2000 bp from so many species you can. Take the areas that show homology and analyse for transcription factor bindning sequences.

http://www.gene-regulation.com/

Try also rVISTA

http://rvista.dcode.org/

http://www.phylofoot.org/