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Web-based Bioinformatics analysis- A road map

Entry Points and General Resources
 
EBI:
  The EMBL - European Bioinformatics Institute (EBI) is a centre for research and services in bioinformatics. The Institute manages databases of biological data including nucleic acid, protein sequences and macromolecular structures.
http://www.ebi.ac.uk

NCBI:
 

What does NCBI do?
Established in 1988 as a national resource for molecular biology information, NCBI creates public
databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminates biomedical information - all for the better understanding of molecular processes affecting human health and disease
http://www.ncbi.nlm.nih.gov/


The Sanger Center:
 

The Sanger Centre is a genome research centre founded by the Wellcome Trust and the Medical
Research Council. Our purpose is to further the knowledge of genomes, particularly through large scale sequencing and analysis.
http://www.sanger.ac.uk/


KEGG Kyoto Encyclopedia of Genes and Genomes
 

Search and compute with KEGG
http://www.genome.ad.jp/kegg/kegg3.html


ExPASy Molecular Biology Server
 

the ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics (SIB). This server is dedicated to the analysis of protein sequences and structures as well as 2-D PAGE
http://www.expasy.ch/


WWW Virtual Library, Biology Division
 

A searchable collection of biology links maintained by Harvard Biological Laboratories.
http://mcb.harvard.edu/BioLinks.html


WWW Virtual Library, Genetics Subdivision
 

Links specific to molecular biology and genetics, maintained by Oak Ridge National Laboratory.
http://www.ornl.gov/TechResources/Human_Genome/genetics.html


EMBnet
 

EMBnet is a science-based group of collaborating nodes throughout Europe and a number of nodes outside Europe.
The combined expertise of the nodes allows EMBnet to provide services to the European molecular biology community which encompasses more than can be provided by a single node.
http://www.embnet.org


GDB Mapping Resources Pages
 

Complete list of genome centers and model organism sites. May not be updated after June 1998.
http://gdbwww.gdb.org/gdb/hgpResources.html


Pour les francophones parmis vous mes amies
  http://bioweb.pasteur.fr/

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If you have a name and you want to find a sequence:
 
NetEntrez
  One-stop shopping for sequences submitted to GenBank. Many search and crossreferene options, maintained by the friendly folk at the NCBI.
http://www.ncbi.nlm.nih.gov/Entrez/

SRS (Sequence Retrieval System)
  A massively crossreferenced index of DNA, protein, 3D structure and function databases.
Conveniently located in Europe.
SRS 7 servers:
http://srs.ebi.ac.uk/
http://downloads.lionbio.co.uk/publicsrs.html

If you have a sequence and you want to find its name:
 
The NCBI BLAST server
 

Sequence homology searching.
http://www.ncbi.nlm.nih.gov/BLAST/


Sanger Centre: H. sapiens, S. cerevisiae, S. pombe, C. elegans, P. falciparum, M. leprae....
  Multiple organisms in various stages of sequencing at the Sanger Center.
http://www.sanger.ac.uk/DataSearch/

Washington University: C elegans, C. briggsae, A. thaliana, H. sapiens
 

Multiple organisms in various stages of sequencing at Wash U.
http://genome.wustl.edu/gsc/gschmpg.html


Stanford: A. thaliana
  BLAST server for various Arabidopsis extracts from GenBank.
http://arabidopsis.org/blast/

EBI
  Here the user will be able to use various sequence database similarity search tools such as Fasta, Blast, MPsrch and Scanps. Interactive as well as email submissions are available for each of these services.
 
http://www.ebi.ac.uk/Tools/similarity.html

http://www.ebi.ac.uk/fasta3/


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Got a Sequence and you want to know eg., its coding region, exon/intron organisation?
 
GRAIL
  GrailEXP is a software package that predicts exons, genes, promoters, polyas, CpG islands, EST similarities, and repetitive elements within DNA sequence. .
http://compbio.ornl.gov/grailexp/

GeneFinder
  This page contains software tools designed to predict putative internal protein coding exons in genomic DNA sequences. Human, mouse and arabidopsis exons are predicted by a program called MZEF (developed by Dr. Michael Zhang). fission yeast exons are predicted by a program called Pombe (developed by Dr. Tim Chen and Dr. Michael Zhang).
http://rulai.cshl.org/tools/genefinder/

GenScan
  http://embnet2.bmc.uu.se:8081/perl/run?script=genscan
Our own service that is up to date

NCSA Biologist's Workbench
  A large collection of sequence and protein analysis tools, designed for interactive use.
http://workbench.sdsc.edu/

PRIMER3
  Automated PCR primer picking at the Whitehead Institute's Web site.
http://www.genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi
.
Promoter analysis sites:
http://www.cbs.dtu.dk/services/Promoter/

http://fastlink.nih.gov/molbio/proscan/index.html

http://www.fruitfly.org/seq_tools/promoter.html

http://genes.mit.edu/McPromoter.html


Transcription factor bindnings site:

The home of TRANSFAC:
http://www.gene-regulation.com/

rVISTA : Vista combines database searches with comparative sequence analysis, reducing the number of false positive predictions by ~95% while maintaining a high sensitivity of the search.
http://rvista.dcode.org/

Got a Sequence: do you want to place it on its right environment?
 
Regulatory Sequence Analysis Tools
  This site provides a series of modular computer programs specifically designed for the detection of regulatory signals in non-coding sequences.
http://liv.bmc.uu.se/rsa-tools/

KEGG (Methabolic pathways and Regulatory pathways)
  Japan
http://www.genome.ad.jp/kegg/kegg2.html

GDB (Genome Database)
  Human maps.
http://gdbwww.gdb.org/

 

Human Gene Map
  UniGene clusters placed on a radiation hybrid map by an international cabal.
http://www.ncbi.nlm.nih.gov/genemap/

Online Mendelian Inheritance in Man
  The definitive guide to known human genes, diseases, and phenotypes. Now integrated into Net Entrez.
http://www3.ncbi.nlm.nih.gov/entrez/query.fcgi?db=OMIM

 

Stanford University Human Map
  A high resolution radiation hybrid map of the human genome.
http://shgc.stanford.edu/

FlyBase
  Drosophila maps (and other information) maintained by University of Indiana.
http://flybase.bio.indiana.edu/

MGD (Mouse Genome Database)
  Definitive repository of mouse mapping information, maintained by Jackson Laboratory.
http://www.jax.org/

SGD (Saccharomyces Genome Database)
  Maps of baker's yeast, maintained at Stanford.
http://genome-www.stanford.edu/Saccharomyces/

YPD (Yeast Protein Database)
  Well-curated compendium of information on yeast enzymes and other proteins.
http://www.proteome.com/

TIGR
  A well-organized database of various microbial genomes, maintained at The Institute for Genome Research.
http://www.tigr.org/

ACEDB
  An online C. elegans database maintained at the Sanger Centre
http://www.acedb.org/

 
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Exploiting the human genome resources
 
Blast the human genome
  http://www.ncbi.nlm.nih.gov/genome/seq/page.cgi?F=HsBlast.html&&ORG=Hs

Ensembl
  Ensembl is a joint project between EMBL-EBI and the Sanger Centre to develop a software system which produces and maintains automatic annotation on eukaryotic genomes.
http://www.ensembl.org/
Human Genome Browser
  by Jim Kent
http://genome.ucsc.edu/cgi-bin/hgGateway?org=human

If you want to find tissue specific Expression
 
Blast your sequence to STACK
  http://www.rfcgr.mrc.ac.uk/Bioinformatics/Databases/stack-help.html

GeneNote
  GeneNote is a database of human genes and their expression profiles in healthy tissues.It is based on Weizmann Institute of Science DNA array experiments, which were performed on the Affymetrix HG-U95 set A-E.

http://genecards.weizmann.ac.il/cgi-bin/genenote/home_page.pl

SymAtlas 0.8.0

  SymAtlas is a web-application for publishing experimental gene functionalization datasets integrated with a flexibly searchable gene-centric database of public and proprietary annotations.
http://symatlas.gnf.org/SymAtlas/


Got a Sequence: is this result already published?

PubMed
  Bibliographic retrieval system, maintained by NCBI. Well-integrated into net Entrez.
http://www.ncbi.nlm.nih.gov/PubMed/


You want to build up your own workbench in bioinformatics?
This a list of links to get information on free available tools and download places.

The Sanger Center
  A collection of software EMBOSS, Alfresco, BLAST, Bioperl, etc
http://www.sanger.ac.uk/Software/

EBI
  A collection of software for different OS
ftp://ftp.ebi.ac.uk/pub/software/

The CCP11 project (Collaborative Computational Project 11)
  Listings of Bioinformatics Programs
http://www.hgmp.mrc.ac.uk/CCP11/

Bioinformatics tools for the MacOSX platform
  Easy to install package containing among others: EMBOSS, NCBI-tools, ClusllW, Primer3 etc
http://www.ebioinformatics.org

Bioinformatics LiveCD for PCs
  Easy to install linux CD containing several Bioinformatics packages.

http://bioknoppix.hpcf.upr.edu/

http://public.vigyaancd.planetmirror.com/


Jim Kents page
  http://www.cse.ucsc.edu/~kent/
   
   This list is maintained by Erik Bongcam-Rudloff, Last update 2005-01-24

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Steves free web site templates

  Listings of Bioinformatics Programs
http://www.hgmp.mrc.ac.uk/CCP11/

Bioinformatics tools for the MacOSX platform
  Easy to install package containing among others: EMBOSS, NCBI-tools, ClusllW, Primer3 etc
http://www.ebioinformatics.org

Bioinformatics LiveCD for PCs
  Easy to install linux CD containing several Bioinformatics packages.

http://bioknoppix.hpcf.upr.edu/

http://public.vigyaancd.planetmirror.com/


Jim Kents page
  http://www.cse.ucsc.edu/~kent/      This list is maintained by Erik Bongcam-Rudloff, Last update 2005-02-24


Steves free web site templates