stssearch

 

Function

Searches a DNA database for matches with a set of STS primers

Description

stssearch searches a DNA sequence database with a set of STS primers and reports expected matches.

stssearchs reads in one or more sequences to be searched. For each pair of primers, it looks for matches between a the primers and the query sequence in either orientation.

Any matches found will be reported. Only one primer need match for it to be reported..

Usage

Here is a sample session with stssearch 


% stssearch 
Searches a DNA database for matches with a set of STS primers
Input sequence(s): tembl:eclac*
Primer file: ../../data/lac.primers
Output file [eclac.stssearch]:

Go to the input files for this example
Go to the output files for this example

Command line arguments 

   Standard (Mandatory) qualifiers:
  [-seqall]            seqall     Sequence database USA
  [-primersfile]       infile     Primer file
  [-outfile]           outfile    Output file name

   Additional (Optional) qualifiers: (none)
   Advanced (Unprompted) qualifiers: (none)
   Associated qualifiers:

   "-seqall" associated qualifiers
   -sbegin1             integer    Start of each sequence to be used
   -send1               integer    End of each sequence to be used
   -sreverse1           boolean    Reverse (if DNA)
   -sask1               boolean    Ask for begin/end/reverse
   -snucleotide1        boolean    Sequence is nucleotide
   -sprotein1           boolean    Sequence is protein
   -slower1             boolean    Make lower case
   -supper1             boolean    Make upper case
   -sformat1            string     Input sequence format
   -sdbname1            string     Database name
   -sid1                string     Entryname
   -ufo1                string     UFO features
   -fformat1            string     Features format
   -fopenfile1          string     Features file name

   "-outfile" associated qualifiers
   -odirectory3         string     Output directory

   General qualifiers:
   -auto                boolean    Turn off prompts
   -stdout              boolean    Write standard output
   -filter              boolean    Read standard input, write standard output
   -options             boolean    Prompt for standard and additional values
   -debug               boolean    Write debug output to program.dbg
   -verbose             boolean    Report some/full command line options
   -help                boolean    Report command line options. More
                                  information on associated and general
                                  qualifiers can be found with -help -verbose
   -warning             boolean    Report warnings
   -error               boolean    Report errors
   -fatal               boolean    Report fatal errors
   -die                 boolean    Report deaths

Standard (Mandatory) qualifiers Allowed values Default
[-seqall]
(Parameter 1)		 Sequence database USA Readable sequence(s) Required
[-primersfile]
(Parameter 2)		 Primer file Input file Required
[-outfile]
(Parameter 3)		 Output file name Output file <sequence>.stssearch
Additional (Optional) qualifiers Allowed values Default
(none)
Advanced (Unprompted) qualifiers Allowed values Default
(none)

Input file format

Input files for usage example

'tembl:eclac*' is a sequence entry in the example nucleic acid database 'tembl'

File: ../../data/lac.primers

PrimA ACCAGACACCCATCAACAG    TATTTATGCCAGCCAGCCAG
PrimB CGAAAGAATAAGAGCAGGCAAG GTAAGAGAAATAGACAGGCGG
PrimC CGTCAGTATCCCCGTTTACAG  TATCGCCAAAATCACCGCC
PrimD AATACGCAAACCGCCTCTCC   TTATCCGCTCACAATTCCACAC
PrimE AATACGCAAACCGCCTCTCC   CACAACCCGCTCACAATTCCA

The primers file consists of three columns separated by tabs or spaces.

The first column is the name of the primer pair.
The second column is the sequence of the first primer.
The third column is the sequence of the second primer.

Output file format

Output files for usage example

File: eclac.stssearch

ECLAC: PrimA PrimerA matched at 532
ECLAC: (rev) PrimA PrimerB matched at 689
ECLAC: PrimB PrimerA matched at 5743
ECLAC: (rev) PrimB PrimerB matched at 5942
ECLAC: PrimC PrimerA matched at 2954
ECLAC: (rev) PrimC PrimerB matched at 3069
ECLAC: PrimD PrimerA matched at 1074
ECLAC: (rev) PrimD PrimerB matched at 1261
ECLAC: PrimE PrimerA matched at 1074
ECLACA: PrimB PrimerA matched at 98
ECLACA: (rev) PrimB PrimerB matched at 297
ECLACI: PrimA PrimerA matched at 484
ECLACI: (rev) PrimA PrimerB matched at 641
ECLACI: PrimD PrimerA matched at 1026
ECLACI: PrimE PrimerA matched at 1026
ECLACY: PrimB PrimerA matched at 1439
ECLACZ: PrimC PrimerA matched at 1668
ECLACZ: (rev) PrimC PrimerB matched at 1783

The output file consists of one line per match. This consists of:

Data files

None.

Notes

None.

References

None.

Warnings

None.

Diagnostic Error Messages

None.

Exit status

None.

Known bugs

None.

See also

Program nameDescription
eprimer3Picks PCR primers and hybridization oligos
primersearchSearches DNA sequences for matches with primer pairs

If you want something that only reports matches of both primer pairs and can find mismatches, use primersearch.

Author(s)

Peter Rice (pmr © ebi.ac.uk)
Informatics Division, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK

History

Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

Comments